A New Enzymatic Method forl-Phenylalanine Synthesis Using Aminoacylase and Phenylalanine Dehydrogenase
نویسندگان
چکیده
منابع مشابه
New Enzymatic Colorimetric Method for the Quantitative Determination of Phenylalanine in Dry-Blood Spots
Phenylketonuria (PKU) is an autosomal recessive disorder, which is characterized by severe mental retardation, microcephaly and seizures. The symptoms of this disease can be prevented if detected soon after birth. Therefore, blood Phenylalanine (Phe) measurement is essential for the early diagnosis, treatment and dietary monitoring of PKU patients. The goal of this research was to introduce a r...
متن کاملA Novel Efficient Method for Extraction and Purification of Recombinant Phenylalanine Dehydrogenase
Growing applications of phenylalanine dehydrogenase (PheDH) enzyme in the medical and pharmaceutical industries encourages researchers to seek simple, fast and economical alternative purification methods. With goal of finding a new technique, the extraction and purification of recombinant Bacillus badius PheDH in polyethylene glycol 6000 (PEG-6000) and ammonium sulfate aqueous two-phase systems...
متن کاملPhenylalanine determined in plasma with use of phenylalanine dehydrogenase and a centrifugal analyzer.
Quantitative determination of plasma phenylalanine (Phe) is essential for the diagnosis of phenylketonuria (PKU) and the control of dietary therapy of PKU patients. We have adapted a spectrophotometric method (Clin Chim Acta 1991;201:95-8) based on phenylalanine dehydrogenase (EC 1.4.1.-) for rapid and accurate determinations of Phe with the Cobas Fara II centrifugal analyzer. The method is bas...
متن کاملThe enzymatic conversion of phenylalanine to tyrosine.
The enzymatic oxidation of phenylalanine to tyrosine has been studied as part of an investigation of the intermediates and enzyme systems involved in the biosynthesis of epinephrine. Womack and Rose (1) indicated that phenylalanine was converted to tyrosine in viva by showing that tyrosine is not essential when sufficient phenylalanine is available. Definite proof was provided by Moss and Schoe...
متن کاملa novel efficient method for extraction and purification of recombinant phenylalanine dehydrogenase
growing applications of phenylalanine dehydrogenase (phedh) enzyme in the medical and pharmaceutical industries encourages researchers to seek simple, fast and economical alternative purification methods. with goal of finding a new technique, the extraction and purification of recombinant bacillus badius phedh in polyethylene glycol 6000 (peg-6000) and ammonium sulfate aqueous two-phase systems...
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ژورنال
عنوان ژورنال: Bioscience, Biotechnology, and Biochemistry
سال: 1997
ISSN: 0916-8451,1347-6947
DOI: 10.1271/bbb.61.1216